Journal: Clinical Cancer Research
Article Title: Emerin Dysregulation Drives the Very-Small-Nuclear Phenotype and Lineage Plasticity That Associate with a Clinically Aggressive Subtype of Prostate Cancer
doi: 10.1158/1078-0432.CCR-24-3660
Figure Lengend Snippet: Patients who are vsnCTC+ have CTCs with decreased EMD expression. A, EMD expression in vsnCTCs by a fluorescence microscope. DAPI (blue), EMD (orange), CK (green), and CD45 (red) staining is shown (scale bar, 10 μm). The vsnCTCs (<8.5 μm, DAPI+/CK+/CD45 − ) were stained with EMD. Arrows, EMD-positive punctate structures. B, EMD expression in individual WBCs, non-vsnCTCs, and vsnCTCs. C, Spearman correlation between EMD expression and the nucleus size of the CTC in patients with mCRPC. D, Spearman correlation between CK expression and the nucleus size of the CTC in patients with mCRPC. *, P < 0.05; ***, P < 0.001; ns, nonsignificant.
Article Snippet: Candidate CTCs were characterized as 4′,6-diamidino-2-phenylindole (DAPI)–positive/cytokeratin (CK)-positive/CD45-negative cells with round or oval nuclei using an upright fluorescence microscope (Eclipse 90i, Nikon Instruments Inc.).
Techniques: Expressing, Fluorescence, Microscopy, Staining